Use of the Fluorescent Dye PicoGreenTM for Quantification of PCR Products after Agarose Gel Electrophoresis
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چکیده
منابع مشابه
Use of the fluorescent dye PicoGreen for quantification of PCR products after agarose gel electrophoresis.
Exact quantification of the amount of amplified polymerase chain reaction (PCR) product is a crucial element in quantitative PCR approaches. Normally such quantification is based on the application of radioactive(2) or fluorochrome-labeled primers (4), HPLC (3) or densitometric-scanning of an agarose gel after staining with dyes like ethidium bromide (1). These quantification techniques often r...
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abstract nowadays, the science of decision making has been paid to more attention due to the complexity of the problems of suppliers selection. as known, one of the efficient tools in economic and human resources development is the extension of communication networks in developing countries. so, the proper selection of suppliers of tc equipments is of concern very much. in this study, a ...
15 صفحه اول[Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification.].
BACKGROUND Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and a researcher-friendly gel electrophoresis-based PCR method for the quantification of mRNA, and the results were compared with those obtained by real-time PCR. METHODS We compared the results of rela...
متن کاملAgarose gel electrophoresis.
MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...
متن کاملAgarose gel electrophoresis.
Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96213bm05